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Testosterone
Blood Testing

Testosterone

Testosterone is a key hormone that supports muscle mass, strength, energy, mood, and metabolic function. In Australia, total testosterone is measured in nmol/L, with typical male reference ranges around 8.3–29 nmol/L. Optimal levels help maintain lean body mass, bone strength, fat metabolism, and long-term vitality.

Tracking testosterone gives valuable insight into how your body manages energy, recovery, and performance. Staying within an optimal range—not just a normal one—supports better wellbeing and longevity. Regular testing helps you identify trends early and adjust lifestyle factors before imbalances occur.

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What is Testosterone?

Testosterone is the primary androgen (male sex hormone) produced mainly in the testes (and in smaller amounts by the ovaries/adrenals) that circulates in the bloodstream and drives multiple processes: muscle-growth and maintenance, bone integrity, fat metabolism, and influence on mood and energy. In men, total testosterone is typically measured in nmol/L and is one of the key biomarkers reflecting androgen status.

Why does it matter for long-term health and wellbeing?

From a preventive and performance-oriented viewpoint, maintaining healthy testosterone levels helps preserve lean muscle mass, supports metabolic efficiency (such as better fat-burning and insulin sensitivity) and helps sustain strength and vitality as we age. Research highlights how muscle mass and strength are closely tied to overall health outcomes and longevity, and testosterone plays a part in those adaptive responses.

Monitoring testosterone gives you a window into your hormonal health and how it may be responding to lifestyle factors like sleep, nutrition, exercise and stress—so you can optimise rather than just react.

What’s an optimal level of Testosterone?

  • Laboratory reference range in Australian adult males: approximately 8.3 to 29 nmol/L.
  • At present there is no universally agreed “optimal” range above and beyond the standard reference, as optimal depends on age, health status and individual goals.
  • For proactive optimisation (rather than disease screening) you might aim for the mid-to-upper part of the reference range, while recognising individual variation and working with trends over time.

(Note: for women, total testosterone reference ranges are much lower and different guidelines apply.)

What influences Testosterone levels?

Many lifestyle, environmental and biological factors impact testosterone levels. Some key influences include:

  • Age: Testosterone tends to peak in early adulthood and gradually declines with age.
  • Body composition and fat-mass: Higher levels of fat, especially visceral fat, and lower lean mass are associated with lower testosterone.
  • Exercise and muscle-building: Strength and resistance training help support healthy testosterone responses and lean mass.
  • Nutrition: Dietary pattern, calorie balance and macro-nutrition (especially protein and healthy fats) influence hormone production.
  • Sleep, stress and recovery: Poor sleep quality, high chronic stress and inadequate recovery can suppress testosterone.
  • Binding-proteins: Sex Hormone Binding Globulin (SHBG) and albumin affect how much testosterone is free vs bound and thus biologically active.

What does it mean if Testosterone is outside the optimal range?

  • If measured testosterone falls near the lower end of the laboratory reference—or below your individual trend—it may signal that hormonal support for muscle, metabolism and energy is less optimal. This doesn’t necessarily imply a disease state, but it does offer a valuable insight for lifestyle optimisation.
  • If testosterone is at the higher end of the range (or elevated beyond the expected range for your age and health status), it may reflect stronger androgenic drive, but also calls for awareness of balance (e.g., how it interacts with recovery, sleep, and other hormones).

In both cases, tracking over time is key: one single value is less actionable than your direction of change and response to lifestyle interventions.

How can I support healthy Testosterone levels?

Here are evidence-based lifestyle strategies to help support testosterone levels:

  • Prioritise resistance and strength training to preserve and build lean muscle mass, which in turn supports testosterone signalling.
  • Ensure sufficient dietary protein and healthy fats (including sources of saturated and mono-/poly-unsaturated fats) as raw materials for hormone production.
  • Maintain healthy body composition—reducing excess adiposity and supporting lean mass helps hormone regulation.
  • Optimise sleep quantity and quality: aim for consistent, adequate sleep and minimise disruptions.
  • Manage stress and recovery: chronic stress can drive hormonal imbalance; include recovery routines, breaks and relaxation.
  • Monitor your trends: regular testing lets you see how your testosterone responds to your habits so you can fine-tune.

This information is provided for general health and wellness purposes only and does not replace medical advice.

References

  1. Handelsman, D. J. et al. Endocrine Society of Australia position statement on male hypogonadism: The reference interval in young men is 7.4–28.0 nmol/L. Med J Aust. 205(4). 
  2. Royal College of Pathologists of Australasia (RCPA). Testosterone – Total. Reference intervals and assay notes.
  3. Gharahdaghi, N., et al. Links Between Testosterone, Oestrogen and Growth Hormone: implications for skeletal muscle mass regulation. Front Physiol. 2020;11:621226.
  4. Green, D. J., et al. Comparing the impacts of testosterone and exercise on lean body mass in older adults. J Gerontol A Biol Sci 2023.
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Biological Age
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Biological age

A reflection of how your body is aging at the cellular level, linked to age risks and longevity.
Red Blood Cell (RBC) Count
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Mean Cell Haemoglobin Concentration (MCHC)
Red Cell Distribution Width (RDW)
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Key blood indicators like hemoglobin, inflammation, and oxygen transport for overall health.
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Assesses cardiovascular health through cholesterol, lipids, and heart-related risk markers.
Cortisol
Dehydroepiandrosterone Sulfate (DHEA-S)
Free Thyroxine (T4)
Free Triiodothyronine (T3)
Thyroid stimulating hormone (TSH)
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Hormone balance

Evaluates hormone levels that impact energy, sleep, mood, and metabolism.
White Blood Cell (WBC) Count
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Immune system

Measures immune activity and inflammatory responses to assess body defenses.
High-Sensitivity C-Reactive Protein (hs-CRP)
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Inflammation status

Tracks signs of chronic or acute inflammation that may affect long-term disease risk.
Bicarbonate
Chloride
Creatinine
Potassium
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Urea
Uric acid
Anion Gap
Estimated Glomerular Filtration Rate (EGFR)
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Assesses kidney health through creatinine and other markers of blood filtration.
Alanine Aminotransferase
Albumin
Alkaline Phosphatase
Aspartate aminotransferase (AST)
Bilirubin
Gamma-glutamyl Transferase (GGT)
Globulin
Total Protein
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Liver health

Measures liver enzymes and proteins responsible for detoxification and metabolic health.
Fasting Glucose
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Haemoglobin A1c (HbA1c) IFCC mmol/m
Haemoglobin A1c (HbA1c) NGSP/DCCT %
HOMA-IR score
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Metabolic health

Evaluates energy processing with glucose, HbA1c, insulin, and related biomarkers.
Magnesium
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Vitamins & minerals

Analyzes essential nutrients that support immunity, energy, and overall wellbeing.
Creatine Kinase
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Muscle strength

Assesses biomarkers related to muscle performance, function, and recovery.
Biological Age
1 marker

Biological age

A reflection of how your body is aging at the cellular level, linked to age risks and longevity.
Red Blood Cell (RBC) Count
Haemoglobin
Haematocrit
Mean Cell Volume (MCV)
Mean Cell Haemoglobin (MCH)
Mean Cell Haemoglobin Concentration (MCHC)
Red Cell Distribution Width (RDW)
Platelet Count
8 markers

Blood health

Key blood indicators like hemoglobin, inflammation, and oxygen transport for overall health.
Cholesterol Ratio
HDL Cholesterol
LDL Cholesterol
Non-HDL Cholesterol
Total Cholesterol
Triglycerides
6 markers

Heart health

Assesses cardiovascular health through cholesterol, lipids, and heart-related risk markers.
Cortisol
Dehydroepiandrosterone Sulfate (DHEA-S)
Free Thyroxine (T4)
Free Triiodothyronine (T3)
Thyroid stimulating hormone (TSH)
5 markers

Hormone balance

Evaluates hormone levels that impact energy, sleep, mood, and metabolism.
White Blood Cell (WBC) Count
Neutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
6 markers

Immune system

Measures immune activity and inflammatory responses to assess body defenses.
High-Sensitivity C-Reactive Protein (hs-CRP)
1 marker

Inflammation status

Tracks signs of chronic or acute inflammation that may affect long-term disease risk.
Bicarbonate
Chloride
Creatinine
Potassium
Sodium
Urea
Uric acid
Anion Gap
Estimated Glomerular Filtration Rate (EGFR)
9 markers

Kidney function

Assesses kidney health through creatinine and other markers of blood filtration.
Alanine Aminotransferase
Albumin
Alkaline Phosphatase
Aspartate aminotransferase (AST)
Bilirubin
Gamma-glutamyl Transferase (GGT)
Globulin
Total Protein
8 markers

Liver health

Measures liver enzymes and proteins responsible for detoxification and metabolic health.
Fasting Glucose
Fasting Insulin
Haemoglobin A1c (HbA1c) IFCC mmol/m
Haemoglobin A1c (HbA1c) NGSP/DCCT %
HOMA-IR score
5 markers

Metabolic health

Evaluates energy processing with glucose, HbA1c, insulin, and related biomarkers.
Magnesium
1 marker

Vitamins & minerals

Analyzes essential nutrients that support immunity, energy, and overall wellbeing.
Creatine Kinase
1 marker

Muscle strength

Assesses biomarkers related to muscle performance, function, and recovery.
Biological Age
1 marker

Biological age

A reflection of how your body is aging at the cellular level, linked to age risks and longevity.
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