For practitioners

Luteinising hormone (LH) testing for Australian practitioners

Luteinising hormone (LH) is the pituitary signal that triggers ovulation and drives testosterone production in the ovaries and testes. Practitioners add it alongside FSH to see where a hormone problem starts: in the gonads, in the pituitary, or with suppression from medication, energy deficit or stress.

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Short answer

Luteinising hormone (LH) is a direct blood measurement of the pituitary signal that triggers ovulation and drives testosterone production in the ovaries and testes. Practitioners use it alongside FSH to locate where a hormone problem starts: the gonads, the pituitary, or suppression from medication, energy deficit or stress. Results depend on cycle day, with the mid-cycle surge reading several times higher.

Luteinising hormone (LH) test facts
Test typeMeasured in a blood sample
Measurements requiredLuteinising hormone (LH) only
Sample and collectionBlood draw at a collection centre, timed to the cycle (day 2 to 5) if the client is cycling
PreparationIf you have periods, book your test for day 2 to 5 of your cycle. Day 1 is the first day of full bleeding.
TurnaroundAbout 3 to 5 business days
Available throughHormone Studies. Available in: Female Hormone Health Test, Perimenopause Test, Menopause Test.
PricingPrice on sign-up
ResultsReturned in the Vively practitioner platform, beside your client's other markers
Ranges

What are the Luteinising hormone (LH) reference and optimal ranges?

Vively optimalTypical lab referenceOutside lab range
MenIU/L
210
Women, day 2 to 5 of cycleIU/L
27
Women, post-menopauseIU/L
1065
Typical lab referenceVively optimal
Men2 to 10 IU/LNo separate optimal range
Women, day 2 to 5 of cycle2 to 7 IU/LNo separate optimal range
Women, post-menopause10 to 65 IU/LNo separate optimal range

Labs differ, so read the result against the range printed on the client's report (RCPA guidance). LH ranges depend on cycle phase, so record the cycle day on the request.

Result checker

Check a client's Luteinising hormone (LH) result

Enter the value from your client's lab report to see where it sits against the typical lab range and the Vively optimal range.

A guide only, not a diagnosis. One result is a starting point: read it with symptoms, history and a repeat test. Labs differ, so check the result against the range on your client's report. Nothing you enter is stored or sent.

How it works

How do you arrange Luteinising hormone (LH) testing through Vively?

  1. Get verified. Practitioners with a recognised qualification or membership (ATMS, NHAA, ANTA, Dietitians Australia, NSA, CMA or Ahpra registration) can request free practitioner access.
  2. Choose a panel. Log in to the practitioner platform and pick a panel that includes Luteinising hormone (LH), or build your own. Add-on pricing shows when you sign up.
  3. Client collects. Your client collects at a partner collection centre, with their request on their phone or printed.
  4. Review results. Results come back to you in the practitioner platform, beside your client's other markers, ready for the follow-up consult.
Panels

Which Vively panels include Luteinising hormone (LH)?

Log in to the practitioner platform to view a panel in full, or build and save your own from 100+ tests and panels.

Evidence

How do you read Luteinising hormone (LH) without an optimal range?

LH has no separate optimal range: it is released in pulses, so read it against FSH, oestradiol or testosterone, SHBG and cycle day.

How to read itPulsatile, with a mid-cycle surge

Single values move more than most hormones, and the mid-cycle surge can reach several times the follicular level.

In cycling womenLH relative to FSH, day 2 to 5

LH running well above FSH is often seen with PCOS, though the 2023 international guideline does not use the ratio for diagnosis because many women with PCOS have normal values.

In menHigh LH with low testosterone: testes

High LH with low testosterone points to the testes, while low or normal LH points to the hypothalamus or pituitary (Endocrine Society of Australia, 2016).

Interpretation

What should Luteinising hormone (LH) be read with?

  • ContextLH about double FSH on day 2 to 5, with raised free testosterone or low SHBG

    Often seen with PCOS and commonly goes with insulin resistance, so check HOMA-IR.

  • ContextMen: low testosterone, high LH

    Points towards reduced testicular output (primary hypogonadism) and needs GP review.

  • ContextMen: low testosterone, low or normal LH

    Points towards central suppression from obesity, opioids, heavy training, illness, or current or past anabolic steroid use.

  • ContextVery high LH and FSH in a cycling woman

    Often a mid-cycle surge caught by timing; repeat on day 2 to 5 before reading further.

  • LowLow LH and FSH, low oestradiol, absent periods

    Possible hypothalamic amenorrhoea from low energy availability (REDs) or major stress.

Before you read it

What can affect a Luteinising hormone (LH) result?

  • Sampling day: the mid-cycle surge reads several times higher than the day 2 to 5 range
  • Pulsatile release: repeat a single borderline value in a man before acting on it
  • The combined pill, depot progestogen, pregnancy and GnRH analogues suppress LH
  • Testosterone therapy, anabolic steroids and SARMs suppress LH, often for months after stopping
  • Opioids, high-dose glucocorticoids, low energy intake and acute illness lower LH
Scope of practice

When should you refer the client to their GP?

  • Men with low testosterone and either high LH or low-normal LH
  • No periods for 3 months or more with low LH and FSH, or suspected low energy availability in an athlete
  • LH in the post-menopausal range in a woman under 40
  • Abnormal LH with headaches, visual change or nipple discharge, which can point to a pituitary cause
Client prep

How should a client prepare for a Luteinising hormone (LH) test?

Copy or print this to send to your client.

Preparing for your Luteinising hormone (LH) test

  1. If you have periods, book your test for day 2 to 5 of your cycle. Day 1 is the first day of full bleeding.
  2. If you are post-menopause, you can test on any day.
  3. Men: book a morning collection so it can be taken with your testosterone sample.
  4. Tell your practitioner if you take the combined oral contraceptive pill, as it lowers LH and your result will reflect the pill.
  5. Take your pathology request to any of 4,000+ collection centres. Bring it printed or on your phone.
  6. Keep taking prescribed medicines as usual unless your doctor tells you otherwise.
  7. Results usually take about 3 to 5 business days and come to your practitioner. Book your results appointment for after that.
In practice

How do practitioners use Luteinising hormone (LH) with clients?

With male clients who train, ask plainly about testosterone, steroid and SARM use before reading a low LH; it is a frequent explanation and the client's GP needs to know. For cycling clients, record the cycle day on the request.

FAQs

Luteinising hormone (LH) FAQs

Can a naturopath, nutritionist or dietitian order an LH test through Vively?

Yes. Add it to any client panel on the Vively practitioner platform. It sits in Hormone Studies and is also in Female Hormone Health Test, Perimenopause Test and Menopause Test. Add-on pricing shows when you sign up. No account yet? Request free access.

Is the LH to FSH ratio useful for PCOS?

Only as supporting context. LH running two to three times FSH on day 2 to 5 is often seen with PCOS, but many women with PCOS have a normal ratio. The 2023 international guideline bases diagnosis on irregular cycles, clinical or biochemical androgen excess, and ovarian morphology or AMH. Read LH with free testosterone, SHBG and HOMA-IR.

Should men have LH tested in the morning?

Yes, on the same morning sample as testosterone, ideally before 10 am. Testosterone is highest in the morning and LH is needed to read it, so pairing them on one draw makes the result interpretable. Because both vary, the Endocrine Society of Australia advises confirming low testosterone on a repeat morning sample before drawing conclusions.

Why is LH tested on day 2 to 5 of the cycle?

Because LH ranges depend on cycle phase, and the mid-cycle surge reads several times higher than the early follicular level. Day 2 to 5, with day 1 as the first day of bleeding, gives a baseline you can read against FSH. A very high LH and FSH in a cycling woman is often a surge caught by timing, so repeat on day 2 to 5 before reading further.

What does a low or normal LH with low testosterone mean in a male client?

It points to the hypothalamus or pituitary rather than the testes, often central suppression from obesity, opioids, heavy training, illness, or current or past anabolic steroid use. Ask plainly about testosterone, steroid and SARM use, since these can suppress LH for months after stopping. LH is pulsatile, so repeat a borderline value, and involve the client's GP.

Sources

References

  1. Royal College of Pathologists of Australasia. RCPA Manual: Luteinising hormone (LH).
  2. Teede HJ, Tay CT, Laven J, et al. Recommendations from the 2023 international evidence-based guideline for the assessment and management of polycystic ovary syndrome. 2023.
  3. Yeap BB, Grossmann M, McLachlan RI, et al. Endocrine Society of Australia position statement on male hypogonadism (part 1). Medical Journal of Australia. 2016. Source
  4. Mountjoy M, Ackerman KE, Bailey DM, et al. 2023 International Olympic Committee consensus statement on relative energy deficiency in sport (REDs). British Journal of Sports Medicine. 2023. Source
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